Journal: Molecular & Cellular Proteomics : MCP
Article Title: Quantitative Age-specific Variability of Plasma Proteins in Healthy Neonates, Children and Adults *
doi: 10.1074/mcp.M116.066720
Figure Lengend Snippet: ELISA validation of differentially expressed proteins. A, GPI-specific phospholipase 1 (GPLD1), B, Periostin (POSTN), C, Alpha-2-macroglobulin (A2M), D, Histidine rich glycoprotein (HRG). E, For comparison, boxplots showing the respective abundance patterns detected from the SWATH data using the extended library (GPLD1 and POSTN) and the local library (A2M and HRG). All error bars are standard deviation (SD). * Denotes significance ≤ 0.05; ** denotes significance ≤ 0.01.
Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA) validation We utilized the following ELISA kits according to the manufacturer's specifications: GPI-specific phospholipase D (GPLD1); (CSB-EL009721HU, Cusabio, Wuhan, China), Periostin (POSTN) (CSB-E16444H, Cusabio, Wuhan, China), Alpha-2-macroglobulin (A2M) (ab108888, Abcam, Cambridge, United Kingdom), Histidine rich glycoprotein (HRG) (CSB-E13159H, Cusabio, Wuhan, China).
Techniques: Enzyme-linked Immunosorbent Assay, Biomarker Discovery, Comparison, Data-independent acquisition, Standard Deviation